Advik Biotech Lab

Plant, Cell, Tissue and Organ Culture

Introduction to PTC
Industrial application of PTC
Plant tissue culture lab organization
Calibration and Handling of laboratory equipment’s
Reagent preparation
Concept of sterilization techniques and surface sterilization
Preparation of Culture Media
Preparation of cotton plugs
Viability testing
Inoculation and culturing of different types of explants
Culture techniques: Callus culture, Embryo culture, Anther culture, Pollen culture, Protoplast isolation and culture
Process of Sub-culturing
Acclimatization methodology
Study of abiotic and biotic stress effect on in vitro raised culture

Phytochemistry and Pharmacognosy of Natural Products

Introduction of natural product
SOP of different instruments
Molarity and Normality Calculation
Extraction procedure through Soxhlet apparatus/steam distillation method
Standard Curve preparation
Qualitative and quantitative analysis of plant secondary metabolites
Estimation of Phenolic/Carbohydrates/Protein through spectrophotometer
Extraction of essential oils
Antimicrobial susceptibility tests
Minimum inhibitory concentration (MIC) of plant extract against bacteria through Disc diffusion method
Synergistic and antagonistic effect of herbal drug
Separation of compound by TLC (Thin layer chromatography)
Separation of herbal extract by Column chromatography

Microbiology

Basics of Microbiology
Good laboratory practices
Concept of basic calculation, solution preparation
Different Sterilization techniques
Media preparation
Culture Techniques
Streak Plate Method
Serial dilution and Pour plate method,
Spread plate method.
Isolation of bacteria from air, water and soil
Isolation of pure bacterial colonies
Isolation of fungus from soil
Staining techniques: Simple staining, Gram staining and Endospore staining
Lactophenol cotton blue staining for fungi.
Biochemical activities of microorganisms: Starch hydrolysis test, IMViC test
Growth curve measurement of bacterial population by turbidometry.
Milk testing
Antibiotic Sensitivity Test.

Bioinformatics

Introduction to bioinformatics,
Conceptual understanding about application of molecular biology
Information Search and Data retrieval: electronic libraries
Tools for web search and data mining
Bioinformatics applications
Bioinformatics tools
Introduction of databases
Major databases in bioinformatics
Data management and analysis
Tools for similarity search and alignments
FASTA and BLAST, DNA/RNA/Protein Sequences: alignments and analysis
Introduction to Phylogenic Studies and Clustal W
Computational modelling - Molecular Docking
ADMET predictions
Advanced analytics
Machine Learning
Natural Language Processing based data science analysis

Pharmaceutical Science

Introduction of pharmaceutical chemistry
General and safety rules for working in Lab
Instrument handling
Concept of calculation and solution preparation
Presence of nitrogen in sample
Presence of sulphur
Presence of halogens group
Presence of esters group
Presence of phenols
Presence of sugar
Presence of non-reducing sugar
Presence of aldehyde
Qualitative analysis of Paracetamol and Aspirin
Presence of ketone
Presence of amide
Limit test of chloride

Biochemistry

Good laboratory practices
Basic instrument handling
Concept of basic calculation
Preparation of reagents, buffers and solutions
Qualitative analysis of sugars
Qualitative analysis of amino acid
Estimation of Saponification value of oils
Estimation of unsaturation fats and oils
Estimation of soluble protein1
Determination of iodine value in fats and oils 1
Analysis of lactose by music test 1
Quantitative estimation of Carbohydrate1
Measurement of blood glucose level